Home  Contacts  Order  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

RiboRuler™ RNA Ladders
For RNA sizing and quantification on native and denaturing gels

Features

• Provide accurate size determination of single-stranded RNA.
• Precise RNA concentration allows for RNA quantification on gels.
• Stable at -20°C for 6 months, minimum.
• Form sharp bands of uniform intensity.

Description & Other Information

Product List:

• RiboRuler™ Low Range RNA Ladder (100-1000 b)
• RiboRuler™ Low Range RNA Ladder, ready-to-use (100-1000 b)
• RiboRuler™ High Range RNA Ladder (200-6000 b)
• RiboRuler™ High Range RNA Ladder, ready-to-use (200-6000 b)

Product Resources (in pdf):

• LabAid™

Frequently Asked Questions (FAQs)

Related Products

RiboRuler™ RNA Ladder, Low Range

The RiboRuler™ Low Range RNA Ladder is a mixture of seven chromatography-purified single-stranded RNA transcripts. The RNA concentration of each ladder band is precisely measured by spectrophotometry (see photos below), which makes this ladder ideal for approximate RNA quantification on gels.
The RiboRuler™ Low Range RNA Ladder is recommended for electrophoresis in the following: native 2% agarose with TAE buffer, denaturing formaldehyde agarose with MOPS buffer, denaturing glyoxal/DMSO agarose with sodium phosphate buffer and denaturing polyacrylamide gel electrophoresis in TBE buffer.
The ladder is supplied with 2X RNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Volume, µl	Applications	Loading, µl/8 mm lane	Range,  bases	Fragments	Agarose,  %	PAGE, %
Conventional>>	SM1831		MSDS (English) MSDS (English-USA) MSDS (German)	100 (5x20)	50	2	100-1000	7	1.7-2.5	4-8
Ready-to-use>>	SM1833		MSDS (English) MSDS (English-USA) MSDS (German)	200 (5x40)	50	4	100-1000	7	1.7-2.5	4-8

Range
7 fragments (in bases): 1000, 800, 600, 400, 300, 200, 100.

RiboRuler™ RNA Ladder, High Range

The RiboRuler™ High Range RNA Ladder is a mixture of eight chromatography-purified single-stranded RNA transcripts. The RNA concentration of each ladder band is precisely measured by spectrophotometry (see photos below), which makes this ladder ideal for approximate RNA quantification on gels.
The RiboRuler™ High Range RNA Ladder is recommended for electrophoresis in the following: native 1% agarose with TAE buffer, denaturing formaldehyde agarose with MOPS buffer and denaturing glyoxal/DMSO agarose with sodium phosphate buffer.
The ladder is supplied with 2X RNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Volume, µl	Applications	Loading, µl/8 mm lane	Range,  bases	Fragments	Agarose,  %	PAGE, %
Conventional>>	SM1821		MSDS (English) MSDS (English-USA) MSDS (German)	100 (5x20)	50	2	200-6000	8	0.8-1.5	-
Ready-to-use>>	SM1823		MSDS (English) MSDS (English-USA) MSDS (German)	200 (5x40)	50	4	200-6000	8	0.8-1.5	-

Range
8 fragments (in bases): 6000, 4000, 3000, 2000, 1500, 1000, 500, 200.

Description
RiboRuler™ RNA ladders are mixtures of chromatography purified single-stranded RNA transcripts, produced from specific templates that contain a fragment of the pTZ19R polylinker and lambda phage fragments. These ladders are ideal for qualitative and quantitave analysis of RNA both in native or denaturing agarose and polyacrylamide gels. An optimized sequence guarantees the migration of the ladder bands according to their actual size in a variety of electrophoretic conditions. Chromatography purified RiboRuler™ RNA ladders are free of degraded RNA and NTPs; the RNA concentration of each ladder band is precisely measured by spectrophotometry, making the RiboRuler™ RNA ladders ideal for approximate RNA quantification on gels.
All RNA ladders are supplied with 2X RNA Loading Dye. The inclusion of ethidium bromide in the 2X RNA Loading Dye allows visualization of the RNA without additional staining of denaturing gels. For better RNA visualization on native gels, additional staining with ethidium bromide is recommended.
Ready-to-use RiboRuler™ RNA ladders are premixed with RNA Loading Dye. After denaturation for 10 min at 70°C and chilling on ice, ready-to-use RiboRuler™ RNA ladders can be directly applied onto the gel (see Protocols for RNA Electrophoresis).
RiboRuler™ RNA ladders (except the ready-to-use versions) are suitable for end-labeling using T4 Polynucleotide Kinase (see protocol) making them ideal for Northern blots.
All RiboRuler™ RNA ladders are aliquoted in smaller volumes to minimize the possibility of contamination during usage.

Storage Buffer (for conventional RNA ladders)
1 mM EDTA (pH 6.0).

Storage and RNA Loading Buffer (for ready-to-use RNA ladders)
47.5% formamide, 0.0125% SDS, 0.0125% bromophenol blue, 0.0125% xylene cyanol FF, 0.0125% ethidium bromide and 0.75 mM EDTA.

2X RNA Loading Dye
95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide and 0.5 mM EDTA.

Recommendations for Loading (see Protocols for RNA Electrophoresis)

• Use the supplied 2X RNA Loading Dye for sample RNA and ladder RNA. Loading equal volumes of sample RNA and ladder RNA is recommended.
• For RiboRuler™ RNA Ladders:
  Use 0.25 µl of RNA ladder per 1 mm of gel lane width.
• Mix 2 µl of RNA ladder and 2 µl of the 2X RNA Loading Dye.
• Heat RNA ladders at 70°C for 10 min.
• Chill on ice and centrifuge briefly prior to loading onto the gel.
• For ready-to-use RiboRuler™ RNA Ladders:
  Use 0.5 µl of ready-to-use RNA ladder per 1 mm of gel lane width.
  • Heat RNA ladders at 70°C for 10 min.
  • Chill on ice and centrifuge briefly prior to loading onto the gel.

Quality Control
RiboRuler™ Low Range RNA Ladders are tested on native agarose and polyacrylamide gels.
RiboRuler™ High Range RNA Ladders are tested on native agarose gels.
RNA concentration is determined spectrophotometrically. The absence of ribonucleases is confirmed by appropriate tests.

Storage and Stability
Store at -20°C for 6 months, or at -70°C for 24 months.
Ready-to-use RiboRuler™ RNA ladders, premixed with 2X RNA Loading Dye, are stable at +4°C for approximately 2 months.
Note
Any RNA, including RiboRuler™ RNA ladders, is extremely sensitive to degradation by ribonucleases. The use of fresh electrophoresis buffer, freshly poured gels, diethyl pyrocarbonate (DEPC)-treated solutions and protective gloves minimizes the possibility of contamination. Gloves are also necessary when solutions containing ethidium bromide are handled.

Related Products

TopVision™ LE GQ Agarose TopVision™ LM GQ Agarose 2X RNA Loading Dye 10X TBE Buffer 50X TAE Buffer T4 Polynucleotide Kinase RiboLock™ RNase Inhibitor Calf Intestine Alkaline Phosphatase (CIAP) DNase I, RNase-free Agarase SP6 RNA Polymerase T3 RNA Polymerase T7 RNA Polymerase RevertAid™ First Strand cDNA Synthesis Kits First Strand cDNA Synthesis Kit T7 Transcription Kit Ribonucleotides for Transcription: ATP, molecular biology grade CTP, molecular biology grade GTP, molecular biology grade UTP, molecular biology grade NTP Set, molecular biology grade Aminoallyl-UTP, molecular biology grade 0.5 M EDTA, pH 8.0 Glycogen, RNA grade DEPC-treated Water

Updated vasario 27, 2008 13:35