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PageBlue™ Protein Staining Solution

 
#R0571 1 liter (for up to 150 mini-gels)

Related Documents (in pdf, ~30-50 KB):

Certificate of Analysis: #R0571
MSDS (English)
MSDS (English-USA)
MSDS (German)

Description
PageBlue™ Protein Staining Solution is a ready-to-use solution for a highly sensitive staining of proteins separated in polyacrylamide gels. PageBlue™ contains Coomassie Brilliant Blue G-250 dye. Due to the property of the dye to form colloidal particles, proteins are preferentially stained without significant staining of the gel matrix. The linear dynamic range of PageBlue™ extends over two orders of magnitude (see picture below) which is superior to Coomassie R-250. The stain does not contain toxic methanol or acetic acid.

Features
  • Ready-to-use.
  • Safe - does not contain methanol or acetic acid.

  • Rapid staining procedure, no destaining required.

  • End point stain, overstaining does not occur.

  • More sensitive than Coomassie Brilliant Blue R-250.

  • Linear dynamic range from 5ng to 500ng.

  • Compatible with:

  • Economical - can be reused up to three times.

Sensitivity
Detects as little as 5ng of protein.

Quality Control
Tested for staining sensitivity in mini-gels using Protein Molecular Weight Marker.

Storage
Store at +4°C to 26°C.

Sensitivity and linear dynamic range of PageBlue™ Protein Staining Solution.
a - beta galactosidase,
b - bovine serum albumin.
Electrophoresis conditions: 12% SDS-PAGE, 0.75 mm gel, well width 3 mm.

 

Staining Protocol for Mini-gels

Fast Protocol:
  1. Washing (only for SDS containing gels):
    • Place the gel into a tank and add 100 ml of deionized or distilled water. 
    • Put the uncovered tank with the gel into a microwave oven and turn on high power for 1 min. Do not boil.
    • Take out the tank from the microwave oven and continue washing with gentle agitation for 4 min.
    • Discard the wash.

Repeat 3 times.

 3x5 min
  1. Staining:
    • Add 20 ml (or enough to cover the gel completely) of PageBlue™ Protein Staining Solution. 
    • Put the uncovered tank into a microwave oven and turn on high power for up to 30 seconds. Do not boil.
    • Take out the tank from the microwave oven and continue staining with gentle agitation for 20 min.
    • Discard the staining solution.
 20 min
  1. Washing:
    • Add 100 ml of deionized or distilled water and rinse for 5 min. 
    • Discard the wash and add 100 ml of deionized or distilled water for gel storage.
 5 min

Total time: 

 25 min for native gels;
40 min for SDS containing gels

Conventional Protocol:
  1. Washing (only for SDS containing gels):
    • Place the gel into a tank and add 100 ml of deionized or distilled water and rinse for 10 min. 
    • Discard the wash.

Repeat 3 times.

 3x10 min
  1. Staining:
    • Add 20 ml (or enough to cover the gel completely ) of PageBlue™ Protein Staining Solution and staining with gentle agitation.
    • Discard the staining solution.
 60 min 
(or overnight)
  1. Washing:
    • Add 100 ml of deionized or distilled water and rinse for 5 min. 
    • Discard the wash and add 100 ml of deionized or distilled water for gel storage.
 5 min

Total time: 

 65 min for native gels;
95 min for SDS containing gels

Note

  • The staining solution can be reused up to 3 times without noticeable decrease in sensitivity

  • Large gels require more staining solution, as gels should be completely immersed in it. When several gels are stained, increase the amount of staining solution accordingly.

  • Washing (step 1) is dedicated to the removal of SDS from the denaturing gels since SDS interferes with the staining. This step can be omitted for native gels.
Related Products
PageRuler™ Prestained Protein Ladder
PageRuler™ Protein Ladder
Prestained Protein Molecular Weight Marker
Protein Molecular Weight Marker
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Updated vasario 27, 2008 11:41