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MassRuler™ DNA Ladders, ready-to-use &
MassRuler™ Express DNA Ladders, ready-to-use
For accurate quantification and sizing of DNA fragments on agarose gels

Features

• Accurate DNA quantification - DNA in the ladder bands is quantified spectrophotometrically.
• MassRuler™ Express DNA Ladders provide fast separation under a wide range of electrophoresis conditions. The band pattern is not affected by the composition of electrophoresis buffer, voltage or gel percentage.
• Ready-to-use - premixed with 6X MassRuler™ DNA Loading Dye for direct loading onto agarose gel.
• Produce sharp bands.
• Easy-to-remember fragment sizes.
• Stable at room temperature for 6 months.

Description & Other Information

Range Selection Guide

Product List:

• MassRuler™ DNA Ladder, Low Range, ready-to-use (80-1031 bp)
• MassRuler™ Express DNA Ladder, LR Forward, ready-to-use (100-1000 bp)
• MassRuler™ Express DNA Ladder, LR Reverse, ready-to-use (100-1000 bp)
• MassRuler™ DNA Ladder, High Range, ready-to-use (1500-10,000 bp)
• MassRuler™ Express DNA Ladder, HR Forward, ready-to-use (1500-10,000 bp)
• MassRuler™ Express DNA Ladder, HR Reverse, ready-to-use (1500-10,000 bp)
• MassRuler™ DNA Ladder, Mix, ready-to-use (80-10,000 bp)
• MassRuler™ Express Forward DNA Ladder Mix, ready-to-use (100-10,000 bp)
• MassRuler™ Express Reverse DNA Ladder Mix, ready-to-use (100-10,000 bp)

Product Resources (in pdf):

• LabAid™

Related Products

MassRuler™ DNA Ladder, Low Range, ready-to-use

Designed for an accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.
Reference band (500 bp) contains the largest amount of material.
MassRuler™ Low Range DNA Ladder, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading,  µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	SM0383		MSDS (English) MSDS (English-USA) MSDS (German)	60.8	2x500	50-200	5-20	80-1031	11	1.7-2.5

Range
11 fragments (in bp): 1031, 900, 800, 700, 600,  500, 400, 300, 200, 100, 80.

MassRuler™ Express DNA Ladder, LR Forward, ready-to-use
MassRuler™ Express DNA Ladder, LR Reverse, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 10ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 1).
MassRuler™ Express DNA Ladders, LR, Forward and Reverse, are supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1263		MSDS (English) MSDS (English-USA) MSDS (German)	28	2x500	50-200	5-20	100-1000	6	0.7-2.0
	#SM1273		MSDS (English) MSDS (English-USA) MSDS (German)

Range
6 fragments (in bp): 1000, 700, 500, 300, 200, 100.

Table 1. Separation Guide for Various Electrophoresis Conditions.

   

MassRuler™ DNA Ladder, High Range, ready-to-use

Designed for an accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.
MassRuler™ High Range DNA Ladder, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM0393		MSDS (English) MSDS (English-USA) MSDS (German)	42.2	2x500	50-200	5-20	1500-10,000	9	0.7-1.2

Range
9 fragments (in bp): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500.

MassRuler™ Express DNA Ladder, HR Forward, ready-to-use
MassRuler™ Express DNA Ladder, HR Reverse, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 15ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 2).
MassRuler™ Express DNA Ladders, HR, Forward and Reverse are supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1243		MSDS (English) MSDS (English-USA) MSDS (German)	28.5	2x500	50-200	5-20	1500-10,000	6	0.7-1.5
	#SM1253		MSDS (English) MSDS (English-USA) MSDS (German)

Range
6 fragments (in bp): 10000, 7000, 5000, 3000, 2000, 1500.

Table 2. Separation Guide for Various Electrophoresis Conditions

   

MassRuler™ DNA Ladder Mix, ready-to-use

Designed for an accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.
Two reference bands (1031 and 500 bp) contain the largest amount of material.
MassRuler™ DNA Ladder Mix, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM0403		MSDS (English) MSDS (English-USA) MSDS (German)	103	2x500	50-200	5-20	80-10,000	20	1.0-1.2

Range
20 fragments (in bp): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80.

MassRuler™ Express Forward DNA Ladder Mix, ready-to-use
MassRuler™ Express Reverse DNA Ladder Mix, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 15ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 3).
MassRuler™ Express DNA Ladder Mixes, Forward and Reverse, are supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications 0.5 µg/lane	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1283		MSDS (English) MSDS (English-USA) MSDS (German)	56.5	2x500	50-200	5-20	100-10,000	12	0.7-1.5
	#SM1293		MSDS (English) MSDS (English-USA) MSDS (German)

Range
12 fragments (in bp): 10000, 7000, 5000, 3000, 2000, 1500, 1000, 700, 500, 300, 200, 100.

Table 3. Separation Guide for Various Electrophoresis Conditions.

   

Description
MassRuler™ DNA Ladders are specially designed for an accurate quantification and sizing of DNA fragments by agarose gel electrophoresis. The integral intensity of each ladder’s fragment is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.
MassRuler™ Express DNA Ladders, Forward and Reverse, are specifically designed for the most accurate quantification of a wide range of DNA fragments. In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size, whereas in the reverse versions, the mass is inversely proportional to fragment’s size.
MassRuler™ Express DNA Ladders (Forward and Reverse) allow fast and reliable DNA quantification in short separation distance over a broad range of voltages, gel percentages and in any electrophoresis buffer (see Tables 1-3 above).
MassRuler™ DNA Ladders are supplied with 6X MassRuler™ DNA Loading Dye for sample DNA.

Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.

6X MassRuler™ DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA.

Recommendations for Loading

• Dilute your DNA sample with the 6X MassRuler™ DNA Loading Dye (supplied with the ladder):
  mix 1 volume of the dye with 5 volumes of the DNA solution.
• Load equal volumes of your DNA sample and MassRuler™ DNA ladder per each lane.
• Do not heat before loading.

Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.

Storage
Store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.

Related Products

TopVision™ LE GQ Agarose TopVision™ LM GQ Agarose 6X MassRuler™ DNA Loading Dye 6X DNA Loading Dye   6X Orange DNA Loading Dye 10X TBE Buffer 50X TAE Buffer Agarase DNA Gel Extraction Kit 0.5 M EDTA, pH 8.0 Water, nuclease-free

Updated balandžio 24, 2008 18:28