Home  Contacts  Order  OEM  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

Spectra™ Multicolor Broad Range Protein Ladder
Broad range protein sizing (10-260 kDa)

		Lot specific pictures: 27614, 29602/0, 32239, 34744, 36669, 38945/4
#SM1841  2x250 µl (for 50 mini gel applications, 10 µl/well or 25 large gel applications, 20 µl/well)
#SM1842  10x250 µl (for 250 mini gel applications, 10 µl/well or 125 large gel applications, 20 µl/well)
Related Documents (in pdf, ~100 KB): Certificate of Analysis: #SM1841, #SM1842 MSDS (English) MSDS (English-USA) MSDS (German) Migration patterns in different electrophoresis conditions (213 KB) LabAid™ (247 KB)

 

Features

• Sharp and stable colors.
• Multicolor - four different colors.
• Ready to load - no need to boil.
• Easy to track on gel.
• Excellent western transfer.

Description
The Spectra™ Multicolor Broad Range Protein Ladder is a 4-color protein standard with 10 prestained recombinant prokaryotic proteins covering a wide range molecular weights from 10 to 260 kDa. Four different chromophores are bound to the proteins producing a brightly colored ladder with an easy-to-remember pattern.
The Spectra™ Multicolor Broad Range Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and for approximate sizing of proteins (1-3).

Applications

• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after Western Blotting.
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Storage Buffer
62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol.

Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting.

Storage
Stable at 4°C for up to 3 months. For long term storage, store at -20°C.

Recommendations for Loading

Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil. Mix gently, but thoroughly, to ensure that the solution is homogeneous. Load the following volumes of the ladder on SDS-polyacrylamide gel: - 10 µl per well for mini gels; - 20 µl per well for large gels. Use the same volumes for Western Blotting. Note Each lot of Spectra™ Multicolor Broad Range Protein Ladder is calibrated against a precisely sized PageRuler™ Unstained Protein Ladder in a Tris-glycine gel and the calculated apparent molecular weights are reported in the product's Certificate of Analysis. Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis.

 

Related Products

DualColor™ Protein Loading Buffer Pack Spectra™ Multicolor High Range Protein Ladder Spectra™ Multicolor Low Range Protein Ladder PageRuler™ Unstained Protein Ladder PageRuler™ Prestained Protein Ladder PageRuler™ Plus Prestained Protein Ladder PageSilver™ Silver Staining Kit PageBlue™ Protein Staining Solution 10X Tris-glycine-SDS 10X Tris-tricine-SDS DTT ProteoJET™ Mammalian Cell Lysis Reagent ProteoJET™ Cytoplasmic and Nuclear Protein Extraction Kit Bradford reagent, ready-to-use Bovine Gamma Globulin Standard Set, ready-to-use Bovine Serum Albumin Standard Set, ready-to-use

References

1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.
2. Burnette, W.N., "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203, 1981.
3. Towbin, H., et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354, 1979.

Updated balandžio 30, 2009 15:29