Spectra™ Multicolor Broad Range Protein Ladder
Broad range protein sizing (10-260 kDa)
Lot specific pictures:
27614, 29602/0#SM1841 2x250 µl
(for 50 mini gel applications, 10 µl/well
or 25 large gel applications, 20 µl/well)Related Documents (in pdf, ~100 KB):
Certificate of Analysis: #SM1841
MSDS (English)
MSDS (English-USA)
MSDS (German)
Migration patterns in different electrophoresis conditions (213 KB)
Features
- Sharp and stable colors.
- Multicolor - four different colors.
- Ready to load - no need to boil.
- Easy to track on gel.
- Excellent western transfer.
Description
The Spectra™ Multicolor Broad Range Protein Ladder is a 4-color protein standard with 10 prestained recombinant prokaryotic proteins covering a wide range molecular weights from 10 to 260 kDa. Four different chromophores are bound to the proteins producing a brightly colored ladder with an easy-to-remember pattern.
The Spectra™ Multicolor Broad Range Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and for approximate sizing of proteins (1-3).
Applications
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
- Monitoring protein transfer onto membranes after Western Blotting.
- Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Composition
62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting.Storage
Stable at 4°C for up to 3 months. For long term storage, store at -20°C.
Recommendations for Loading
- Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil.
- Mix gently, but thoroughly, to ensure that the solution is homogeneous.
- Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 10 µl per well for mini gels;
- 20 µl per well for large gels.
Use the same volumes for Western Blotting.Note
- Each lot of Spectra™ Multicolor Broad Range Protein Ladder is calibrated against a precisely sized PageRuler™ Unstained Protein Ladder in a Tris-glycine gel and the calculated apparent molecular weights are reported in the product's Certificate of Analysis.
- Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.
- A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis.
Related Products
- ProteoJET™ Mammalian Cell Lysis Reagent
- ProteoJET™ Cytoplasmic and Nuclear Protein Extraction Kit
- Bradford reagent, ready-to-use
- Bovine Gamma Globulin Standard Set, ready-to-use
- Bovine Serum Albumin Standard Set, ready-to-use
- Protein Standard Solution
- PageRuler™ Unstained Protein Ladder
- Unstained Protein Molecular Weight Marker
- PageSilver™ Silver Staining Kit
- PageBlue™ Protein Staining Solution
- Protein Loading Buffer Pack
- 10X Tris-glycine-SDS
- 10X Tris-glycine
- 10X Tris-tricine-SDS
- DTT
- Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.
- Burnette, W.N., "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203, 1981.
- Towbin, H., et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354, 1979.
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Updated balandžio 03, 2008 09:22
