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Prestained Protein Molecular Weight Marker

		Lot specific pictures: 21726, 23251, 24549, 26792
#SM0441	2x250 µl (for 100 mini gel applications, 5 µl/well or 50 large gel applications, 10 µl/well)
Related Documents (in pdf, ~100 KB): Certificate of Analysis: #SM0441 MSDS (English) MSDS (English-USA) MSDS (German) LabAid™

 

Features

• Size range: 20-120 kDa.
• Ready-to-use - supplied in a loading buffer for direct loading on gels.

Description
The Prestained Protein Molecular Weight Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis (1), verification of Western transfer efficiency and approximate sizing of proteins. The marker is a mixture of 6 proteins with the apparent molecular weights from 20 kDa to 120 kDa. All proteins of the marker are covalently coupled to a blue chromophore.
The Prestained Protein Molecular Weight Marker can be used in Western blotting on PVDF, nylon and nitrocellulose membranes.

Applications

• Monitoring of protein migration during SDS- polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes during Western blotting (see Western Blotting protocol).
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.

Composition
Approximately 0.2 mg/ml of each protein in 62.5  mM Tris-HCl (pH 7.5 at 25°C), 1  mM EDTA, 2% SDS, 10  mM DTT, 1.5  mM NaN₃ and 33% glycerol.

Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage
Store at -20°C.

Recommendations for Loading

Thaw the marker either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. Mix gently, but thoroughly, to ensure that the solution is homogeneous. Load the following volumes of the marker on SDS-polyacrylamide gel: - 5 µl per well for mini-gels, 3 µl per well for blots; - 10 µl per well for large gels, 6 µl per well for blots. Note The indicated loading volume is recommended for gels with a thickness of 0.75 mm. For thicker gels, the loading volume should be increased. Each lot of Prestained Protein Molecular Weight Marker is calibrated against a precisely sized Unstained Protein Molecular Weight Marker in Tris-glycine gel and the calculated apparent molecular weights are reported in the product’s Certificate of Analysis. Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis.

 

Related Products

Loading Buffer Pack Unstained Protein Molecular Weight Marker PageBlue™ Protein Staining Solution PageRuler™ Prestained Protein Ladder PageRuler™ Plus Prestained Protein Ladder 10X Tris-glycine-SDS Buffer 10X Tris-tricine-SDS Buffer Protein Standard Solution DTT

Reference

1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.

Updated vasario 06, 2008 16:37