Unstained Protein Molecular Weight Marker
Lot specific pictures:
14249, 16471,
18717, 20339,
22126, 23933,
25176#SM0431 2x1000 µl
(for 400 mini gel applications, 5 µl/well
or 200 large gel applications, 10 µl/well)Related Documents (in pdf, ~100 KB):
Certificate of Analysis: #SM0431
MSDS (English)
MSDS (English-USA)
MSDS (German)
LabAid™
Features
- Size range: 14.4-116 kDa.
- Supplied in a loading buffer for direct loading on gels.
- Sharp bands.
Description
The Unstained Protein Molecular Weight Marker is designed for precise sizing of proteins by SDS-polyacrylamide gel electrophoresis (1). It is a mixture of 7 proteins (14.4-116 kDa ) which appear as sharp bands after SDS-polyacrylamide gel electrophoresis when stained with PageBlue™ Protein Staining Solution or by other protein staining methods.
The Unstained Protein Molecular Weight Marker can be used in Western blotting on PVDF, nylon and nitrocellulose membranes.Application
Accurate protein sizing on SDS-polyacrylamide gels.Composition
0.1-0.2 mg/ml of each protein in 62.5 mM Tris-HCl (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 50 mM DTT, 30 mM NaCl, 1 mM NaN3, 0.01% bromophenol blue and 50% glycerol.Quality Control
Tested in SDS-polyacrylamide gel electrophoresis.Storage
Store at -20°C.
Recommendations for Loading
It is recommended to divide the marker into several aliquots to avoid contamination of the stock solution.
- Thaw the marker either at room temperature or at 37-40°C for a few minutes.
- Mix gently, but thoroughly, to ensure that the solution is homogeneous.
- Remove the required amount of the marker from the stock solution and transfer it to a clean tube with a screw cap.
- Heat this tube at 95°C for 5 minutes to completely denature proteins.
- Chill on ice.
- Load the following volumes of the marker on SDS-polyacrylamide gel:
- 5 µl per well for mini-gels;
- 10 µl per well for large gels.Note
- The indicated loading volume is recommended for gels with a thickness of 0.75 mm. For thicker gels, the loading volume should be increased.
- Once denatured the marker can be further used just after the thawing by omitting the steps 3-5 in the above protocol.
- A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis.
Recommendations for Detection
- After the electrophoresis is complete, stain the gel with PageBlue™ Protein Staining Solution, see protocol.
- For silver staining (9), the volume of Unstained Protein Molecular Weight Marker used should be decreased up to 10-fold.
Related Products
Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.
- Fowler, A.V., Zabin, I., The amino acid sequence of beta-galactosidase of Escherichia coli, Proc. Natl. Acad. Sci. USA, 74, 1507-1510, 1977.
- Brown, J.R., Structure of bovine serum albumin, Fed. Proc., 34, 591, 1975.
- Warner, R.C., Egg proteins, Proteins, II A., 435, (Neurath, H., Bailey, K., eds.), Academic Press, N.Y., 1954.
- Castellino, F.J., Barker, R., Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry, Biochemistry, 7, 2207-2217, 1968.
- Unpublished results.
- Dayhoff, M., Atlas of Protein Sequence and Structure, vol.4, National Biomedical Research Foundation, Silver Spring, M.D.,1969.
- Jolles, P., Lysozymes: A chapter of molecular biology, Angew. Chem., Intl. Edit., 8, 227-294, 1969.
- Sorense, B.K., et al., Silver Staining of Proteins on Electroblotting Membranes and Intensification of Silver Staining of Proteins Separated by Polyacrylamide Gel Electrophoresis, Analytical Biochemistry, 304, 33-41, 2002.
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Updated vasario 06, 2008 16:37
