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FastRuler™ DNA Ladders, ready-to-use
For fast (8-14 min) DNA sizing and quantification in high throughput (48-96-well) short run agarose gels

Features

• Designed for high throughput analysis on 48-96-well agarose gels.
• Fast separation (8-14 min), short separation distance (10-20 mm).
• Ready-to-use - premixed with a DNA loading dye for direct loading.
• Produce sharp bands.
• Easy-to-remember fragment sizes and concentrations.
• Suitable for approximate quantification of PCR products or other DNA fragments.
• Stable at room temperature for 6 months.

Description & Other Information

Range Selection Guide

Product List:

• FastRuler™ Ultra Low Range DNA Ladder, ready-to-use (10-200 bp)
• FastRuler™ Low Range DNA Ladder, ready-to-use (50-1500 bp)
• FastRuler™ Middle Range DNA Ladder, ready-to-use (100-5000 bp)
• FastRuler™ High Range DNA Ladder, ready-to-use (500-10000 bp)

Product Resources (in pdf):

• LabAid™

Related Products

FastRuler™ DNA Ladder, Ultra Low Range, ready-to-use

Designed for fast sizing and approximate quantification of double-stranded DNA in 48-96-well high throughput gels and in conventional agarose gels.
DNA marker contains dephosphorylated DNA fragments and is ideal for forward 5'-end labeling with T4 Polynucleotide Kinase (see protocol below).
FastRuler™ Ultra Low Range DNA Ladder, ready-to-use, is supplied with 6X Orange DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1233		MSDS (English) MSDS (English-USA) MSDS (German)	22.2	2x500	50-333	3-20	10-200	5	4.0

Range 5 fragments (in bp): 200, 100, 50, 20, 10.
Performance in 96-well format gel electrophoresis. 10 µl/lane, 4% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min. 1 - 200 bp; 2 - 150 bp; 3 - 100 bp; 4 - 75 bp; 5 - 50 bp; 6 - 35 bp; 7 - 25 bp M - FastRuler™ Ultra Low Range DNA Ladder, ready-to-use		Time course of band separation. 20 µl/lane, 4% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min.

Protocol for Forward Radioactive 5'-termini Labeling using T4 Polynucleotide Kinase

1. Prepare the following reaction mixture:

   FastRuler™ Ultra Low Range DNA Ladder, ready-to-use	2 µl
   10X Reaction Buffer A (supplied with the enzyme)	2 µl
   [gama-32P]-ATP or [gama-33P]-ATP	2 µl (3.3 pmol/µl)
   Water, nuclease-free	13 µl
   T4 Polynucleotide Kinase	1 µl (10 u)

2. Incubate at 37°C for 30 minutes.
3. Add 1 µl 0.5 M EDTA, pH 8.0, and extract with an equal volume of chloroform.
4. Purify the labeled DNA on Sephadex™ G-50 column.

FastRuler™ DNA Ladder, Low Range, ready-to-use

Designed for fast sizing and approximate quantification of double-stranded DNA in 48-96-well high throughput gels and in conventional agarose gels.
FastRuler™ Low Range DNA Ladder, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1103		MSDS (English) MSDS (English-USA) MSDS (German)	20	2x500	50-333	3-20	50-1500	5	2.0

Range 5 fragments (in bp): 1500, 850, 400, 200, 50.
Performance in 96-well format gel electrophoresis. 10 µl/lane, 2% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min. 1 - 1000 bp; 2 - 700 bp; 3 - 400 bp; 4 - 300 bp; 5 - 200 bp; 6 - 150 bp; 7 - 100 bp M - FastRuler™ Low Range DNA Ladder, ready-to-use		Time course of band separation. 20 µl/lane, 2% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min.

FastRuler™ DNA Ladder, Middle Range, ready-to-use

Designed for fast sizing and approximate quantification of double-stranded DNA in 48-96-well high throughput gels and in conventional agarose gels.
FastRuler™ Middle Range DNA Ladder, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1113		MSDS (English) MSDS (English-USA) MSDS (German)	20	2x500	50-333	3-20	100-5000	5	1.0

Range 5 fragments (in bp): 5000, 2000, 850, 400, 100.
Performance in 96-well format gel electrophoresis. 10 µl/lane, 1% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min. 1 - 5000 bp; 2 - 3000 bp; 3 - 2000 bp; 4 - 1500 bp; 5 - 1000 bp; 6 - 700 bp; 7 - 500 bp M - FastRuler™ Middle Range DNA Ladder, ready-to-use		Time course of band separation. 20 µl/lane, 1% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min.

FastRuler™ DNA Ladder, High Range, ready-to-use

Designed for fast sizing and approximate quantification of double-stranded DNA in 48-96-well high throughput gels and in conventional agarose gels.
FastRuler™ High Range DNA Ladder, ready-to-use, is supplied with 6X MassRuler™ DNA Loading Dye.

Ladder format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, ng/µl	Volume, µl	Applications	Loading, µl/lane	Range,  bp	Fragments	Agarose,  %
Ready-to-use>>	#SM1123		MSDS (English) MSDS (English-USA) MSDS (German)	20	2x500	50-333	3-20	500-10,000	5	1.0

Range 5 fragments (in bp): 10000, 4000, 2000, 1000, 500.
Performance in 96-well format gel electrophoresis. 10 µl/lane, 1% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min. 1 - 10000 bp; 2 - 7000 bp; 3 - 5000 bp; 4 - 4000 bp; 5 - 3000 bp; 6 - 2000 bp; 7 - 1000 bp M - FastRuler™ High Range DNA Ladder, ready-to-use		Time course of band separation. 20 µl/lane, 1% TopVision™ LE GQ Agarose, 1X TBE, 7 V/cm, 14 min.

Description
FastRuler™ DNA ladders are specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48-well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladders are mixtures of five blunt-ended chromatography-purified individual DNA fragments that are easily identified after the 8-14 min run on the appropriate agarose gels in a short separation distance (10-20 mm). Supplied with the 6X MassRuler™ DNA Loading Dye or with the 6X Orange DNA Loading Dye for the sample DNA.
FastRuler™ Ultra Low Range DNA Ladder, ready-to-use, contains dephosphorylated DNA fragments and is ideal for forward 5'-end labeling with T4 Polynucleotide Kinase (see protocol).

Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.

6X MassRuler™ DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA.

Storage and Loading Buffer (for FastRuler™ Ultra Low Range)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.

6X Orange DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.

Recommendations for Loading

• For accurate DNA quantification:
  • dilute your DNA sample with the DNA loading dye (supplied with the ladder): mix 1 volume of the dye with 5 volumes of the DNA solution;
  • load different volumes of FastRuler™ DNA Ladders (as indicated in the pictures). Loading equal volumes of the DNA ladder and your sample per each lane is important;
  • if necessary, adjust the concentration of your sample such that the expected amount of material loaded is approximately equal to that of a DNA ladder band of the nearest size.
• Vortex gently before use.
• Do not heat before loading.

Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.

Storage
Store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.

Related Products

TopVision™ LE GQ Agarose TopVision™ LM GQ Agarose 6X MassRuler™ DNA Loading Dye 6X Orange DNA Loading Dye 10X TBE Buffer 50X TAE Buffer T4 Polynucleotide Kinase Agarase DNA Gel Extraction Kit ATP 0.5 M EDTA, pH 8.0 Water, nuclease-free

Updated balandžio 24, 2008 18:15