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Express DNA Ladders, 100-20000 bp
 For fast sizing and quantification of broad range (100-20,000 bp) DNA fragments under a wide variety of electrophoresis conditions

Features

Description & Other Information

Range Selection Guide

Product List:

Product Resources (in pdf):

Related Products

ZipRuler™ Express DNA Ladder Set, ready-to-use

Designed for fast and accurate broad range (100-20,000 bp) DNA sizing and quantification on agarose gels under various electrophoresis conditions (see Table 1).
ZipRuler™ Express DNA Ladder Set, ready-to-use, is supplied with 6X Orange DNA Loading Dye.

More about ZipRuler™ DNA ladders.

ZipRuler™ Express DNA Ladder Set Ladder
format		 Catalog
# 		Certificate
of Analysis
(in pdf)		 Product
resources
(in pdf)		 Concentration,
µg/µl 		Amount,
µg 		Applications Loading,
µg(µl)/lane 		Range,
 bp 		Fragments Agarose, 
%
ZipRuler™ Express DNA Ladder 1 Ready-to-use>> #SM1373

MSDS (English)
MSDS (English-USA)
MSDS (German)

 0.1 50 100-200 0.25-0.5(2.5-5) 100-10000 9 0.7-2.0
ZipRuler™ Express DNA Ladder 2 200-20000

  
Range
ZipRuler™ Express DNA Ladder 1 (9 fragments in bp):  10000,  5000, 3000, 2000, 1200, 850, 500, 300, 100.
ZipRuler™ Express DNA Ladder 2 (9 fragments in bp):  20000, 7000, 4000, 2500, 1500, 1000, 700, 400, 200.

Table 1. ZipRuler™ Express DNA Ladder Separation Guide for Various Electrophoresis Conditions.

MassRuler™ Express DNA Ladder, LR Forward, ready-to-use
MassRuler™ Express DNA Ladder, LR Reverse, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 10ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 2).
MassRuler™ Express DNA Ladders, LR, Forward and Reverse, are supplied with 6X MassRuler™ DNA Loading Dye.

More about MassRuler™ DNA ladders.

Ladder
format		 Catalog
# 		Certificate
of Analysis
 (in pdf)		 Product
resources
 (in pdf)		 Concentration,
ng/µl 		Volume,
µl 		Applications
0.5 µg/lane 		Loading,
µl/lane 		Range,
 bp 		Fragments Agarose, 
%
Ready-to-use>> #SM1263 MSDS (English)
MSDS (English-USA)
MSDS (German)		 28 2x500 50-200 5-20 100-1000 6 0.7-2.0
#SM1273 MSDS (English)
MSDS (English-USA)
MSDS (German)


Range
6 fragments (in bp): 1000, 700, 500, 300, 200, 100.

Table 2. Separation Guide for Various Electrophoresis Conditions.

   

MassRuler™ Express DNA Ladder, HR Forward, ready-to-use
MassRuler™ Express DNA Ladder, HR Reverse, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 15ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 3).
MassRuler™ Express DNA Ladders, HR, Forward and Reverse are supplied with 6X MassRuler™ DNA Loading Dye.

More about MassRuler™ DNA ladders.

Ladder
format		 Catalog
# 		Certificate
of Analysis
 (in pdf)		 Product
resources
 (in pdf)		 Concentration,
ng/µl 		Volume,
µl 		Applications
0.5 µg/lane 		Loading,
µl/lane 		Range,
 bp 		Fragments Agarose, 
%
Ready-to-use>> #SM1243 MSDS (English)
MSDS (English-USA)
MSDS (German)		 28.5 2x500 50-200 5-20 1500-10,000 6 0.7-1.5
#SM1253 MSDS (English)
MSDS (English-USA)
MSDS (German)


Range
6 fragments (in bp): 10000, 7000, 5000, 3000, 2000, 1500.

Table 3. Separation Guide for Various Electrophoresis Conditions

   

MassRuler™ Express Forward DNA Ladder Mix, ready-to-use
MassRuler™ Express Reverse DNA Ladder Mix, ready-to-use

Designed for the most accurate quantification of a wide range of DNA fragments. In the forward version, the mass of each DNA fragment is directly proportional to the fragment size, e.g., 10 µl of the ladder contain respectively 100, 70, 50, 30, 20, 15ng of DNA, whereas in the reverse version, the mass is inversely proportional to fragment’s size.
The band pattern is not affected by the composition of electrophoresis buffer, voltage and gel percentage (see Table 4).
MassRuler™ Express DNA Ladder Mixes, Forward and Reverse, are supplied with 6X MassRuler™ DNA Loading Dye.

More about MassRuler™ DNA ladders

Ladder
format		 Catalog
# 		Certificate
of Analysis
 (in pdf)		 Product
resources
 (in pdf)		 Concentration,
ng/µl 		Volume,
µl 		Applications
0.5 µg/lane 		Loading,
µl/lane 		Range,
 bp 		Fragments Agarose, 
%
Ready-to-use>> #SM1283 MSDS (English)
MSDS (English-USA)
MSDS (German)		 56.5 2x500 50-200 5-20 100-10,000 12 0.7-1.5
#SM1293 MSDS (English)
MSDS (English-USA)
MSDS (German)


Range
12 fragments (in bp): 10000, 7000, 5000, 3000, 2000, 1500, 1000, 700, 500, 300, 200, 100.

Table 4. Separation Guide for Various Electrophoresis Conditions.

   

GeneRuler™ Express DNA Ladder
GeneRuler™ Express DNA Ladder, ready-to-use
O'GeneRuler™ Express DNA Ladder, ready-to-use

Designed for fast separation (in 5-15 min at 23 V/cm) under a variety of electrophoresis conditions. The band pattern is not affected by the composition of electrophoresis buffer, voltage or gel percentage.
GeneRuler™ Express DNA Ladders are supplied with 6X DNA Loading Dye.
O'GeneRuler™ Express DNA Ladder, ready-to-use, is supplied with 6X Orange DNA Loading Dye.

More about GeneRuler™ and O'GeneRuler™ DNA ladders.

Express DNA
ladders		 Ladder
format		 Catalog
# 		Certificate
of Analysis
(in pdf)		 Product
resources
(in pdf)		 Concentration,
µg/µl 		Amount,
µg 		Applications
0.5 µg/lane 		Loading amount,
µg(µl)/lane 		Range,
 bp 		Fragments Agarose, 
%
GeneRuler™ Express Conventional>> SM1551 MSDS (English)
MSDS (English-USA)
MSDS (German)		 0.5 50 100 0.5 (1) 100-5000 9 1.7-2.5
SM1552 250 (5x50) 500
GeneRuler™ Express Ready-to-use>> SM1553 0.1 50 100 0.5 (5)
O'GeneRuler™ Express SM1563 MSDS (English)
MSDS (English-USA)
MSDS (German)
   

Range
9 fragments (in bp): 5000, 3000, 2000, 1500, 1000, 750, 500, 300, 100.
Fast separation of GeneRuler™ Express DNA Ladder at high voltage (23 V/cm)
Electrophoresis conditions: 0.5 µg/lane, 1% TopVision™ LE GQ Agarose, 1X TAE, 23 V/cm, 5-15 min.


Express DNA Ladders,
flyer in pdf, 124 KB

Description
Express DNA ladders are specifically designed for fast electrophoresis under a wide range of electrophoresis conditions. The band pattern is not affected by the composition of electrophoresis buffer, gel percentage or high voltage. Express DNA ladders allow for rapid and accurate sizing and quantification of broad range (100-20,000 bp) DNA fragments. See Tables 1-4 above for guides for separation of Express DNA ladder's under various electrophoresis conditions.
Express DNA ladders supplement such DNA ladder groups as MassRuler™ DNA ladders, GeneRuler™ and O’GeneRuler™ DNA ladders, and compose ZipRuler™ Express DNA Ladders group.

Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.

Storage
Store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.

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Updated balandžio 24, 2008 18:37