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Electrophoresis Buffers

10X Tris-glycine-SDS

 1X formulation Applications
#B46 1 litre 25 mM Tris,
192 mM glycine,
0.1% SDS,
pH 8.3 		Denaturing polyacrylamide gel electrophoresis of proteins (1).

Related Documents (in pdf, ~20-50 KB):

Certificate of Analysis: #B46
MSDS (English)
MSDS (English-USA)
MSDS (German)

10X Tris-glycine

 1X formulation Applications
#B47 1 litre 25 mM Tris,
192 mM glycine,
pH 8.3 		Native polyacrylamide gel electrophoresis of proteins.

Related Documents (in pdf, ~20-50 KB):

Certificate of Analysis: #B47
MSDS (English)
MSDS (English-USA)
MSDS (German)
10X Tris-tricine-SDS  1X formulation Applications
#B48 1 litre 100 mM Tris,
100 mM tricine,
0.1% SDS,
pH 8.3 		Denaturing polyacrylamide gel electrophoresis of low molecular weight proteins and peptides (2).

Related Documents (in pdf, ~20-50 KB):

Certificate of Analysis: #B48
MSDS (English)
MSDS (English-USA)
MSDS (German)
50X TAE (Tris-acetate-EDTA) 1X formulation Applications
#B49 1 litre 40 mM Tris,
20 mM acetic acid,
1 mM EDTA 		Nucleic acid agarose and polyacrylamide gel electrophoresis; agarose and polyacrylamide gel preparation.

Related Documents (in pdf, ~20-50 KB):

Certificate of Analysis: #B49
MSDS (English)
MSDS (English-USA)
MSDS (German)
10X TBE (Tris-borate-EDTA) 1X formulation Applications
#B52 1 litre 89 mM Tris,
89 mM boric acid,
2 mM EDTA 		Nucleic acid agarose and polyacrylamide gel electrophoresis; agarose and polyacrylamide gel preparation.

Related Documents (in pdf, ~20-50 KB):

Certificate of Analysis: #B52
MSDS (English)
MSDS (English-USA)
MSDS (German)

Quality Control
Tested for the absence of deoxyribonucleases, ribonucleases and proteases.

Note
  • Buffer concentrates should be diluted to a working concentration of 1X before use.
  • For each electrophoresis fresh 1X buffer should be used.

References

  1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of Bacteriophage T4, Nature, 227, 680-685, 1970.

  2. Shagger, H., and G.von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem., 166, 368-379, 1987.
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Updated balandžio 24, 2008 18:11