Conventional Phage and Plasmid DNA Markers, 8-1118 bp
For analysis of linear double-stranded small DNA fragments in agarose and polyacrylamide gelsFeatures
- Recommended for sizing and approximate quantification of small DNA fragments.
- Produce sharp bands.
- Conventional versions (supplied in storage (TE) buffer) can be labeled radioactively with T4 Polynucleotide Kinase or with Klenow Fragment, exo-, see protocols.
- Ready-to-use versions are stable at room temperature for 6 months.
Description & Other Information
Range Selection Guide
Product List:
- pBR322 DNA/BsuRI (HaeIII) Marker, 5 (8-587 bp)
- pUC Mix Marker, 8 (19-1118 bp)
- pUC Mix Marker, 8, ready-to-use (19-1118 bp)
- PhiX174 DNA/BsuRI (HaeIII) Marker, 9 (72-1353 bp)
- PhiX174 DNA/BsuRI (HaeIII) Marker, 9, ready-to-use (72-1353 bp)
- PhiX174 DNA/HinfI Marker, 10 (24-726 bp)
- pBR322 DNA/AluI Marker, 20 (11-908 bp)
- pBR322 DNA/AluI Marker, 20, ready-to-use (11-908 bp)
- pUC19 DNA/MspI (HpaII) Marker, 23 (26-501 bp)
- pUC19 DNA/MspI (HpaII) Marker, 23, ready-to-use (26-501 bp)
Product Resources (in pdf):
pBR322 DNA/BsuRI (HaeIII) Marker, 5
Recommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose and non-denaturing polyacrylamide gels.
pBR322 DNA is digested to completion with BsuRI, purified and dissolved in storage buffer.
pBR322 DNA/BsuRI (HaeIII) Marker is supplied with 6X DNA Loading Dye.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%PAGE,
%Conventional>> #SM0271 0.5 50 100 0.5 (1) 8-587 22 2.5 5.0
Range
22 fragments (in bp): 587**, 540**, 502, 458**, 434, 267, 234, 213, 192, 184, 124, 123, 104, 89, 80, 64, 57, 51, 21, 18, 11, 8 (oblique fragments comprise 5.4%).Note
- Bands indicated** form unusual pattern in polyacrylamide gels.
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.
pUC Mix Marker, 8
pUC Mix Marker, 8, ready-to-useRecommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose and non-denaturing polyacrylamide gels.
pUC19 DNA and pUC57 DNA are individually completely digested with MspI and DraI+HindIII respectively, purified and dissolved in a storage buffer. All digests are mixed.
pUC Mix Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%PAGE,
%Conventional>> #SM0301 MSDS (English)
MSDS (English-USA)
MSDS (German)0.5 50 100 0.5 (1) 19-1118 17 1.7 5.0 #SM0302 250 (5x50) 500 Ready-to-use>> #SM0303 0.1 50 100 0.5 (5)
Range
17 fragments (in bp): 1118, 881, 692, 501**, 489**, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26, 19 (oblique fragments comprise 0.9%).Note
- Bands indicated** form unusual pattern in polyacrylamide gels.
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.
PhiX174 DNA/BsuRI (HaeIII) Marker, 9
PhiX174 DNA/BsuRI (HaeIII) Marker, 9, ready-to-useRecommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose and non-denaturing polyacrylamide gels.
PhiX174 DNA is digested to completion with BsuRI, purified and dissolved in storage buffer.
PhiX174 DNA/BsuRI (HaeIII) Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%PAGE,
%Conventional>> #SM0251 MSDS (English)
MSDS (English-USA)
MSDS (German)0.5 50 100 0.5 (1) 72-1353 11 1.7 5.0 #SM0252 250 (5x50) 500 Ready-to-use>> #SM0253 0.1 50 100 0.5 (5)
Range
11 fragments (in bp): 1353, 1078, 872, 603, 310**, 281**, 271**, 234, 194, 118, 72.Note
Bands indicated** form unusual pattern in polyacrylamide gels.
PhiX174 DNA/HinfI Marker, 10
Recommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose and non-denaturing polyacrylamide gels.
PhiX174 DNA is digested to completion with HinfI, purified and dissolved in storage buffer.
PhiX174 DNA/HinfI Marker is supplied with 6X DNA Loading Dye.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%PAGE,
%Conventional>> #SM0261 0.5 50 100 0.5 (1) 24-726 21 2.5 5.0
Range
21 fragments (in bp): 726, 713, 553**, 500, 427**, 417**, 413**, 311, 249, 200, 151, 140, 118, 100, 82, 66, 66, 48, 42, 40, 24 (oblique fragments comprise 1.9%).Note
- Bands indicated** form unusual pattern in polyacrylamide gels.
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.
pBR322 DNA/AluI Marker, 20
pBR322 DNA/AluI Marker, 20, ready-to-useRecommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose gels.
pBR322 DNA is digested to completion with AluI, purified and dissolved in storage buffer.
pBR322 DNA/AluI Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%Conventional>> #SM0121 0.5 50 100 0.5 (1) 11-908 17 1.7 Ready-to-use>> #SM0123 0.1 0.5 (5)
Range
17 fragments (in bp): 908, 659, 656, 521, 403, 281, 257, 226, 100, 90, 63, 57, 49, 46, 19, 15, 11 (oblique fragments comprise 5.9%).Note
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.
- Loading the marker in 5% polyacrylamide gels is not recommended due to anomalous migration of 659, 656, 403 and 257 fragments.
pUC19 DNA/MspI (HpaII) Marker, 23
pUC19 DNA/MspI (HpaII) Marker, 23, ready-to-useRecommended for sizing and approximate quantification of linear double-stranded small DNA fragments in agarose and non-denaturing polyacrylamide gels.
pUC19 DNA is digested to completion with MspI, purified and dissolved in storage buffer.
pUC19 DNA/MspI (HpaII) Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.
Marker
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications
0.5 µg/laneLoading,
µg(µl)/laneRange,
bpFragments Agarose,
%PAGE,
%Conventional>> #SM0221 0.5 50 100 0.5 (1) 26-501 13 1.7 5.0 #SM0222 250 (5x50) 500 Ready-to-use>> #SM0223 0.1 50 100 0.5 (5)
Range
13 fragments (in bp): 501**, 489**, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26 (oblique fragment comprises makes 1.0%).Note
- Bands indicated** form unusual pattern in polyacrylamide gels.
- The shortest fragment (oblique) is not visible in standard electrophoresis. Fragment length is predicted by computer analysis of respective DNA sequence.
Description
Conventional phage and plasmid DNA markers are recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels.
Phage or plasmid DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. DNA fragments contain various ends (blunt or sticky) depending on the restriction enzyme used for marker’s preparation.
pUC Mix, PhiX174 DNA/BsuRI, pBR322 DNA/AluI and pUC19 DNA/MspI markers are also available in ready-to-use versions - premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels. All markers are supplied with 6X DNA Loading Dye.Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
- Use the 6X DNA Loading Dye (supplied with the marker) for your sample and marker DNA: mix 1 volume of the dye with 5 volumes of the DNA solution.
- Apply the ready-to-use conventional phage and plasmid DNA marker:
- 0.2 µl (0.2-0.4 µg) per 1 mm of an agarose gel lane width;
- 1-2 µl (0.1-0.2 µg) per 1 mm of a polyacrylamide gel lane width.
- Use 0.1 µg (0.2 µl) of the conventional phage and plasmid DNA marker per 1mm agarose gel lane width:
- prepare the following mixture for loading on agarose gel:
- 1 µl of DNA marker (0.5 µg)
- 1 µl of 6X DNA Loading Dye
- deionized water up to 6 µl
- vortex gently before use;
- apply the prepared amount (6 µl) of DNA marker on a 5 mm lane of agarose gel.
- Use 0.2 µg (0.4 µl) of the conventional phage and plasmid DNA marker per 1mm polyacrylamide gel lane width:
- prepare the following mixture for loading on polyacrylamide gel:
- 2 µl of DNA marker (1 µg)
- 0.5 µl of 6X DNA Loading Dye
- deionized water up to 3 µl
- vortex gently before use;
- apply the prepared amount (3 µl) of DNA marker on a 5 mm lane of polyacrylamide gel.
- Do not heat before loading.
Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.Storage
Store at -20°C.
Ready-to-use versions store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.
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Updated balandžio 24, 2008 18:42
