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Conventional Lambda DNA Markers, 15-48502 bp
For analysis of linear double-stranded large DNA fragments in agarose gels

Features

• Recommended for sizing and approximate quantification of large DNA fragments.
• Produce sharp bands.
• Conventional versions (supplied in storage (TE) buffer) can be labeled radioactively with T4 Polynucleotide Kinase or with Klenow Fragment, exo-, see protocols.
• Ready-to-use versions are stable at room temperature for 6 months.

Description & Other Information

Range Selection Guide

Product List:

• Lambda DNA/EcoRI Marker, 1 (3530-21226 bp)
• Lambda DNA/HindIII Marker, 2 (125-23130 bp)
• Lambda DNA/HindIII Marker, 2,  ready-to-use (125-23130 bp)
• Lambda DNA/EcoRI+HindIII Marker, 3 (125-21226 bp)
• Lambda DNA/EcoRI+HindIII Marker, 3,  ready-to-use (125-21226 bp)
• Lambda - pUC Mix Marker, 4 (74-19329 bp)
• Lambda DNA/Eco47I (AvaII) Marker, 13 (23-8126 bp)
• Lambda DNA/Eco91I (BstEII) Marker, 15 (117-8453 bp)
• Lambda DNA/Eco130I (StyI) Marker, 16 (74-19329 bp)
• Lambda Mix Marker, 19 (1503-48502 bp)
• Lambda DNA/PstI Marker, 24 (15-11501 bp)

Product Resources (in pdf):

• LabAid™

Related Products

Lambda DNA/EcoRI Marker, 1

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with EcoRI, purified and dissolved in storage buffer.
Lambda DNA/EcoRI Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0281		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	3530-21226	6	0.7

Range
6 fragments (in bp): 21226*, 7421, 5804, 5643, 4878, 3530*.

Note
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.

Lambda DNA/HindIII Marker, 2
Lambda DNA/HindIII Marker, 2,  ready-to-use

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with HindIII, purified and dissolved in storage buffer.
Lambda DNA/HindIII Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0101		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	125-23130	8	1.0
	#SM0102				1250 (25x50)	2500
Ready-to-use>>	#SM0103			0.1	250 (5x50)	500	0.5 (5)

Range
8 fragments (in bp): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.

Note
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.

Lambda DNA/EcoRI+HindIII Marker, 3
Lambda DNA/EcoRI+HindIII Marker, 3,  ready-to-use

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with EcoRI and HindIII, purified and dissolved in storage buffer.
Lambda DNA/EcoRI+HindIII Markers are supplied with 6X DNA Loading Dye.
Ready-to-use version - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0191		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	125-21226	13	1.0
	#SM0192				1250 (25x50)	2500
Ready-to-use>>	#SM0193			0.1	250 (5x50)	500	0.5 (5)

Range
13 fragments (in bp): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125 (oblique fragment comprises 0.3%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragment (oblique) is not visible in standard electrophoresis. Fragment length is predicted by computer analysis of respective DNA sequence.

Lambda - pUC Mix Marker, 4

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is completely digested with Eco130I and Eco81I, pUCGK57 DNA is completely digested with HindIII and dissolved in a storage buffer. All digests are mixed.
Lambda - pUC Mix Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0291		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	50	100	0.5 (1)	74-19329	6	1.0

Range
6 fragments (in bp): 19329*, 7743, 5526, 4254*, 3280, 2690, 2322, 1882, 1489, 1150, 925, 697, 421, 74 (oblique fragment comprises 0.1%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragment (oblique) is not visible in standard electrophoresis. Fragment length is predicted by computer analysis of respective DNA sequence.

Lambda DNA/Eco47I (AvaII) Marker, 13

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with Eco47I, purified and dissolved in storage buffer.
Lambda DNA/Eco47I (AvaII) Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM1051		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	23-8126	36	1.5

Range
36 fragments (in bp): 8126, 6555, 6442, 3676, 2606, 2555, 2134, 2005, 1951, 1611*, 1420, 1284, 985, 974, 894, 597, 590, 513, 511, 433, 398, 345, 310, 308, 272, 242, 215, 151, 88, 73, 67, 45, 42, 32, 29*, 23 (oblique fragments comprise 2.7%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.

Lambda DNA/Eco91I (BstEII) Marker, 15

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with Eco91I, purified and dissolved in storage buffer.
Lambda DNA/Eco91I (BstEII) Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0111		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	117-8453	14	1.0

Range
14 fragments (in bp): 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117 (oblique fragments comprise 0.7%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.

Lambda DNA/Eco130I (StyI) Marker, 16

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with Eco130I, purified and dissolved in storage buffer.
Lambda DNA/Eco130I (StyI) Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0161		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	74-19329	11	1.0

Range
11 fragments (in bp): 19329*, 7743, 6223, 4254*, 3472, 2690, 1882, 1489, 925, 421, 74 (oblique fragment comprises 0.2%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragment (oblique) is not visible in standard electrophoresis. Fragment length is predicted by computer analysis of respective DNA sequence.

Lambda Mix Marker, 19

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Samples of lambda DNA are completely digested with the appropriate restriction enzymes, filled in with Klenow fragment and dissolved in a storage buffer. All digests are mixed.
Lambda Mix Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0231		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	1503-48502	14	0.7

Range
14 fragments (in bp): 48502, 38416, 33498, 29946, 24508, 23994, 19397*, 17053, 15004, 12220, 10086, 8614*, 8271, 1503 (oblique fragment comprises 0.6%)

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragment (oblique) is not visible in standard electrophoresis. Fragment length is predicted by computer analysis of respective DNA sequence.

Lambda DNA/PstI Marker, 24

Recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with PstI, purified and dissolved in storage buffer.
Lambda DNA/PstI Marker is supplied with 6X DNA Loading Dye.

Marker format	Catalog #	Certificate of Analysis (in pdf)	Product resources (in pdf)	Concentration, µg/µl	Amount, µg	Applications 0.5 µg/lane	Loading, µg(µl)/lane	Range,  bp	Fragments	Agarose,  %
Conventional>>	#SM0361		MSDS (English) MSDS (English-USA) MSDS (German)	0.5	250 (5x50)	500	0.5 (1)	15-11501	29	1.5

Range
29 fragments (in bp): 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159, 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15 (oblique fragments comprise 2.3%).

Note

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated* may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min and then cooling on ice for 3 min.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequence.

Description
Conventional lambda DNA markers are recommended for sizing of linear double-stranded large DNA fragments in agarose gels.
Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for marker’s preparation.
Lambda DNA/HindIII Marker and Lambda DNA/EcoRI+HindIII Marker are available in ready-to-use versions - premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose and polyacrylamide gels.
All markers are supplied with 6X DNA Loading Dye.

Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.

6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.

Recommendations for Loading

• Use the 6X DNA Loading Dye (supplied with the marker) for your sample and marker DNA: mix 1 volume of the dye with 5 volumes of the DNA solution.
• Apply the ready-to-use conventional lambda DNA marker:
  • 1 µl (0.1 µg) per 1 mm of an agarose gel lane width;
  • 1-2 µl (0.1-0.2 µg) per 1 mm of a polyacrylamide gel lane width.
• Use 0.1 µg (0.2 µl) of the conventional lambda DNA marker per 1mm agarose gel lane width:
  • prepare the following mixture for loading on agarose gel:
    • 1 µl of DNA marker (0.5 µg)
    • 1 µl of 6X DNA Loading Dye
    • deionized water up to 6 µl
  • vortex gently before use;
  • apply the prepared amount (6 µl) of DNA marker on a 5 mm lane of agarose gel.
•  Use 0.2 µg (0.4 µl) of the conventional lambda DNA marker per 1mm polyacrylamide gel lane width:
  • prepare the following mixture for loading on polyacrylamide gel:
    • 2 µl of DNA marker (1 µg)
    • 0.5 µl of 6X DNA Loading Dye
    • deionized water up to 3 µl
  • vortex gently before use;
  • apply the prepared amount (3 µl) of DNA marker on a 5 mm lane of polyacrylamide gel.
• Heat at 65°C for 5 min before loading.

Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.

Storage
Store at -20°C.
Ready-to-use versions store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.

Related Products

TopVision™ LE GQ Agarose TopVision™ LM GQ Agarose 6X DNA Loading Dye 50X TAE Buffer T4 Polynucleotide Kinase Klenow Fragment Agarase DNA Gel Extraction Kit ATP dNTP Set 0.5 M EDTA, pH 8.0 Water, nuclease-free

Updated balandžio 24, 2008 18:26