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Bradford Reagent, ready-to-use
Fast and easy protein quantification 

#R1271	1 liter (for 1000 standard test tube assays or 4000 standard microplate assays)
Related Documents (in pdf, ~200 KB): Certificate of Analysis: #R1271 MSDS (English) MSDS (English-USA) MSDS (German) Flyer

 

Features

• Ready-to-use formulation - no dilution required.
• Rapid color development - 5 minutes.
• Broad working concentration range - 1-2000 µg/ml.
• Compatible with a variety of substances present in samples, including solvents, salts, detergents, thiols, reducing and chelating agents.

Description
Bradford Reagent, ready-to-use, is a convenient 1X concentrated dye reagent for rapid and accurate estimation of total protein concentration in solution. The Bradford protein assay relies on the formation of complexes between Coomassie Brilliant Blue G-250 dye and proteins in solution. The binding of the dye to the protein causes a shift in the absorption maximum of the dye from 465 nm to 595 nm (1, 2). Unknown protein concentrations are determined by reference of their absorptions to a series of standard protein dilutions. The quantification procedure is simple and rapid. The dye binding process is complete in approximately 5 min with color stability for 1 hour. The working range is 1-2000 µg/ml of protein. Depending on the protein concentration, standard or microassay procedures can be used. The assay can be performed in test tubes or microplates.

Application
Rapid and accurate estimation of protein concentration in solution.

Quality Control
Functionally tested for accurate determination of various protein concentrations.

Storage
Store protected from light at 4°C for one year.

Figure 1. Standard curve obtained using Bradford reagent, ready-to-use, in a standard assay.
BSA - bovine serum albumin.
BGG - bovine gamma globulin.

Figure 2. Standard curve obtained using Bradford reagent, ready-to-use, in a microassay.
BSA - bovine serum albumin.
BGG - bovine gamma globulin.

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References

1. Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248-54, 1976.
2. Sedmak, J.J., Grossberg, S.E., A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G-250, Anal. Biochem., 79, 544-52, 1977.