6X TriTrack™ DNA Loading Dye
#R1161 5x1 ml Related Documents (in pdf, ~30-50 KB):
Certificate of Analysis: #R1161
MSDS (English)
MSDS (English-USA)
MSDS (German)
Features
Visual three-color tracking of DNA migration during electrophoresis.
- No DNA masking during gel exposure to UV light.
Electrophoresis of tracking dyes in 6X TriTrack™ DNA Loading Dye.Description
The 6X TriTrack™ DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. The optimized solution contains three dyes: bromophenol blue, xylene cyanol FF and orange G for visual three-color tracking of DNA migration during electrophoresis. The presence of glycerol in the solution ensures that the same sample forms a layer at the bottom of the well; the EDTA included in the solution binds divalent metal ions and inhibits metal dependent nucleases.Composition of 6X Solution
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 0.15% orange G, 60% glycerol, 60 mM EDTA.
Note
In 1% agarose gels bromophenol blue co-migrates with ~300 bp DNA, while xylene cyanol FF co-migrates with ~4000 bp DNA. Orange G co-migrates with the smallest fragments, i.e. ~50 bp.
- For the migration rates of dyes in agarose and polyacrylamide gels see tables.
Quality Control
Tested for DNA sample preparation prior to agarose gel electrophoresis.Storage
Store at room temperature +4°C up to 12 months.
For longer periods, store at -20°C.
Usage Recommendations
- Add 1 volume of 6X TriTrack™ DNA Loading Dye to 5 volumes of DNA sample.
- Mix well, spin down and load.
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Updated vasario 01, 2008 09:30
