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6X DNA Loading Dye

#R0611	5x1 ml
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis: #R0611 MSDS (English) MSDS (English-USA) MSDS (German)

 

Features

• Visual two-color tracking of DNA migration during electrophoresis.
• No DNA masking during gel exposure to UV light.

Electrophoresis of tracking dyes in 6X DNA Loading Dye

Description
The 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. The optimized solution contains two dyes, bromophenol blue and xylene cyanol FF, for visual tracking of DNA migration during electrophoresis. The presence of glycerol in the solution ensures that the same sample forms a layer at the bottom of the well; the EDTA included in the solution binds divalent metal ions and inhibits metal dependent nucleases.

Composition of 6X Solution
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60 mM EDTA.

Note

• In 1% agarose gels bromophenol blue co-migrates with ~300 bp DNA, while xylene cyanol FF co-migrates with ~4000 bp DNA.
• For the migration rates of dyes in agarose and polyacrylamide gels see tables.

Quality Control
Tested for DNA sample preparation prior to agarose gel electrophoresis.

Storage
Store at room temperature +4°C up to 12 months.
For longer periods, store at -20°C.

Usage Recommendations

Add 1 volume of 6X DNA Loading Dye to 5 volumes of DNA sample. Mix well, spin down and load.

 

Related Products

TopVision™ LE GQ Agarose TopVision™ LM GQ Agarose 10X TBE Buffer 50X TAE Buffer Agarase DNA Markers/Ladders DNA Gel Extraction Kit

Updated vasario 01, 2008 09:30